Influence of peroperative lavage solutions on peritoneal defence mechanisms in vitro

Objective: To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly‐used lavage solutions. Design: Experimental study. Setting: Laboratories, The Netherlands. Material: Cultured human peritoneal mesothelial cells and isolated PMN...

Full description

Saved in:
Bibliographic Details
Published in:The European journal of surgery 1999-11, Vol.165 (11), p.1066-1071
Main Authors: van Westreenen, Mireille, Mul, Frederick J. P., Pronk, Apollo, Hoynck van Papendrecht, Arthur A. G. M., Diepersloot, Rob J. A., Roos, Dirk, Leguit, Piet
Format: Article
Language:English
Subjects:
Anti-Infective Agents, Local - pharmacology
Biological and medical sciences
Cell Survival
Cells, Cultured
Epithelial Cells
Humans
Hydrogen Peroxide - pharmacology
Isotonic Solutions - pharmacology
Liver, biliary tract, pancreas, portal circulation, spleen
Medical sciences
Neutrophils - drug effects
Neutrophils - physiology
Peritoneal Lavage
Peritoneum - cytology
Peritoneum - drug effects
Peritoneum - physiology
Povidone-Iodine - pharmacology
Respiratory Burst
Sodium Hypochlorite - pharmacology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the digestive system
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: To find out the in vitro reaction of mesothelial cells and polymorphonuclear leucocytes (PMN) to incubation with seven commonly‐used lavage solutions. Design: Experimental study. Setting: Laboratories, The Netherlands. Material: Cultured human peritoneal mesothelial cells and isolated PMN. Intervention: Incubation of cells with clinically used lavage solutions (sodium chloride, Hartmann's solution, povidone‐iodine, Dakin's solution, taurolidine, chlorhexidine, and hydrogen peroxide). Main outcome measures: Activation of monolayers of mesothelial cells and PMN measured by release of oxygen free radicals (chemiluminescence) and interleukin (IL)‐8 concentrations and toxic effects measured by morphology, release of lactate dehydrogenase, failure of the restriction of the passage of inulin, and incorporation of propidium iodide. Results: All solutions activated and killed mesothelial cells and PMN to some extent; the more concentrated the solution the greater the effect on these cells. Conclusion: Lavage solutions both poison and stimulate mesothelial cells and neutrophils, and some solutions are more potent than others. Copyright © 1999 Taylor and Francis Ltd.
ISSN:1102-4151
1741-9271
DOI:10.1080/110241599750007919