Circadian variation in mitotic index of primary spermatogonia in the adult frog (Rana esculenta)

Circadian rhythmicity in the average proliferation rate of the primary spermatogonial cell crop in the frog testis was studied by the stathmokinetic method. Colchicine was used to specifically block cells after they enter metaphase. The in vivo mitotic activity of primary spermatogonia was studied i...

Full description

Saved in:
Bibliographic Details
Published in:Bollettino di zoologia 1987-01, Vol.54 (1), p.87-89
Main Authors: Minucci, Sergio, di Matteo, Loredana, di Meglio, Maria, Rastogi, Rakesh K.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Freshwater
Fundamental and applied biological sciences. Psychology
Mitotic index
Non mammalian vertebrate reproduction
Primary spermatogonia
Rana esculenta
Rana esculenta
Circadian variation
Vertebrates: reproduction
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Circadian rhythmicity in the average proliferation rate of the primary spermatogonial cell crop in the frog testis was studied by the stathmokinetic method. Colchicine was used to specifically block cells after they enter metaphase. The in vivo mitotic activity of primary spermatogonia was studied in three different periods of the year and was analyzed at 8 time points of the 24-hour day. Circadian variations were suggested in all three periods of the year. Mitotic activity of the primary spermatogonia was also analyzed in vitro at 16 time points through a 48-hour experimental period. Daily rhythmic fluctuations in the division rate of primary spermatogonia were observed. Thus, the proportion of cells in mitosis within the primary spermatogonial cell crop of the frog testis exhibits not only significant seasonal variations (as reported elsewhere), but also a daily rhythmic variation. The percent difference between low and high values of mitotic circadian rhythm varies with season.
ISSN:0373-4137
DOI:10.1080/11250008709355562