Anatomical and physiological changes of in vitro-propagated Vriesea imperialis (Bromeliaceae) in the function of sucrose and ventilated containers

• Published in: Plant biosystems 2020-01, Vol.154 (1), p.87-99
• Main Authors: Martins, João Paulo Rodrigues, Rodrigues, Luiz Carlos de Almeida, Conde, Lorenzo Toscano, Gontijo, Andreia Barcelos Passos Lima, Falqueto, Antelmo Ralph
• Format: Article
• Language: English
• Subjects: Bromeliad; Cell culture; Cell walls; Containers; Gas exchange; Lignin; Osmotic stress; physiological disturbance; Physiology; plant anatomy; plant physiology; plant tissue culture; Polyvinyl chloride; Stomata; Sucrose; Sugar; Ventilation; Vriesea
• ISSN: 1126-3504; 1724-5575
• DOI: 10.1080/11263504.2019.1635223

Under in vitro culture conditions, plants may present physiological and anatomical disorders, which can interfere negatively after ex vitro transfer. The aim of this investigation was to analyze the impacts of natural ventilation and sucrose supply on the anatomy and physiology of Vriesea imperialis. Plants previously grown in vitro were transferred to culture medium containing 0, 15, 30 or 45 g L −1 sucrose. Three different culture container sealing systems were tested: lids with a green filter (81.35 gas exchanges per day), yellow filter (13.09 gas exchanges per day) or lids with a yellow filter covered with three layers of transparent polyvinylchloride (PVC) film (blocking fluent gas exchange). Sucrose concentrations influenced thickness, lignin and suberin deposition of exodermis cell wall. The modifications verified in leaves, such as higher density of stomata and trichome scales, showed that sucrose can induce osmotic stress in the plants. Photomixotrophic conditions, using containers with intermediate rate of gas exchange (yellow filter) and with 15-30 g L −1 sucrose, produced an improvement in the growth traits and did not induce anatomical and physiological disturbances.