Betulinic acid protects against N-nitrosodimethylamine-induced redox imbalance in testes of rats

Objectives: N-nitrosodimethylamine (NDMA) is known to elicit carcinogenic activity in the liver and kidney of animals. There is a dearth of information of its effect in testis. This study evaluated the protective role of betulinic acid (BA) against NDMA-induced redox imbalance in testes of rats. Met...

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Bibliographic Details
Published in:Redox report : communications in free radical research 2017-11, Vol.22 (6), p.556-562
Main Authors: Adeleke, Gbadebo E., Adaramoye, Oluwatosin A.
Format: Article
Language:English
Subjects:
Animals
antioxidant
Antioxidants - metabolism
Dimethylnitrosamine - toxicity
Lipid Peroxidation - drug effects
Liver - drug effects
Male
Oxidation-Reduction - drug effects
oxidative stress
Pentacyclic Triterpenes
phase I and II enzymes
Rats
Rats, Wistar
Sperm quality
Testis - drug effects
Testis - metabolism
toxicant
Triterpenes - pharmacology
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Summary:Objectives: N-nitrosodimethylamine (NDMA) is known to elicit carcinogenic activity in the liver and kidney of animals. There is a dearth of information of its effect in testis. This study evaluated the protective role of betulinic acid (BA) against NDMA-induced redox imbalance in testes of rats. Methodology: Twenty-four male rats were assigned into four groups and treated with normal saline, BA, NDMA and [BA+NDMA]. BA (25 mg/kg) was given for 14 days, while NDMA (5 mg/kg) was given on days 7 and 12. Results: Administration of NDMA significantly increased the weight and relative weight of testes by 51 and 71%, respectively, while treatment with BA attenuated the weight-gain. Furthermore, NDMA decreased the sperm count, motility and live-dead ratio by 57, 36 and 37%, respectively, and increased total sperm abnormality by 56%. However, BA attenuated the changes in the spermiogram of NDMA-treated rats. NDMA significantly decreased the activities of antioxidative enzymes, follicle-stimulating and luteinizing hormones, while testicular levels of thiobarbituric acid reactive substances and total cholesterol were increased. Also, NDMA increased the activities of aniline hydroxylase and aminopyrine-N-demethylase. Supplementation with BA attenuated NDMA-induced alteration in these biochemical indices. Conclusion: BA protects against NDMA-induced redox imbalance via activation of antioxidative pathway.
ISSN:1351-0002
1743-2928
DOI:10.1080/13510002.2017.1322750