Micropropagation of recalcitrant-seeded Ekebergia capensis Sparrm. using in vitro nodal segments and roots

The present study reports the development of in vitro micropropagation protocols for Ekebergia capensis Sparrm., a species that produces unstorable recalcitrant (non-orthodox) seeds. Suitable protocols were established for shoots generated from nodal segments excised from in vitro-germinated seedlin...

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Bibliographic Details
Published in:The journal of horticultural science & biotechnology 2009-01, Vol.84 (1), p.87-91
Main Authors: Hajari, E., Berjak, P., Pammenter, N.W., Watt, M.P.
Format: Article
Language:English
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Summary:The present study reports the development of in vitro micropropagation protocols for Ekebergia capensis Sparrm., a species that produces unstorable recalcitrant (non-orthodox) seeds. Suitable protocols were established for shoots generated from nodal segments excised from in vitro-germinated seedlings, and for adventitious shoots generated from intact in vitro-germinated roots. Difficulties were experienced when attempts were made to micropropagate germplasm from established saplings. The protocols developed have important applications for the conservation (cryopreservation and clonal multiplication) of germplasm of this species.
ISSN:1462-0316
2380-4084
DOI:10.1080/14620316.2009.11512485