Effect of probiotication on antioxidant and antibacterial activities of pomegranate juices from sour and sweet cultivars

• Published in: Natural product research 2011-02, Vol.25 (3), p.288-297
• Main Authors: Fazeli, M.R, Bahmani, S, Jamalifar, H, Samadi, N
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibacterial activity; antibacterial properties; antioxidant activity; antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Biphenyl Compounds - chemistry; cultivars; fermentation; growth retardation; juices; Lactobacillus casei; Lactobacillus casei - physiology; Microbial Sensitivity Tests; Picrates - chemistry; Plant Exudates - chemistry; pomegranate; pomegranates; probiotication; Probiotics; Pseudomonas aeruginosa; Punicaceae - chemistry
• ISSN: 1478-6427; 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2010.495068

There is an increasing interest in using pomegranate juice as a natural antioxidant rather than synthetic compounds. In this study, the antioxidant capacities of probioticated and nonprobioticated aril juices of sweet (SWV) and sour (SV) pomegranate cultivars were determined by two different methods: ferric reducing antioxidant power (FRAP) and 1,1-diphenyl 2-picrylhydrazyl assay. Total counts of Lactobacillus casei GG increased by about 3 log in SWV and 2 log in SV juices after incubation for 48 h. Probiotication improved the antioxidant activity of SWV juice from 74.4% to 91.82%, and SV juice from 82.64% to 97.8%. Based on the FRAP value, the reducing power of the probioticated pomegranate juices was also much stronger than the nonprobioticated juices. The FRAP values for SWV and SV probioticated juices were 97.34 and 120.7 mmol L⁻¹, respectively, which were notably higher than 85.87 and 93.4 mmol L⁻¹ for SWV and SV nonprobioticated juices. Both fermentated and nonfermentated juices exhibited a potent and wide-spectrum antibacterial effect, with the highest activity against Pseudomonas aeruginosa. SV juice showed wider zones of growth inhibition. The results of this study verify for the first time that probiotication of SWV and SV pomegranate juices can add to their beneficial antioxidant activities.