Novel thermostable and alkalitolerant amylase production by Geobacillus stearothermophilus HP 3

This study reports the production and characterisation of a novel thermostable alkaline amylase from the newly isolated Geobacillus stearothermophilus HP 3. The optimum temperature and pH for enzyme production was 55°C and 9.0, respectively. The amylase powder obtained from the culture filtrate by p...

Full description

Saved in:
Bibliographic Details
Published in:Natural product research 2012-09, Vol.26 (17), p.1626-1630
Main Author: Selim, Samy A.
Format: Article
Language:English
Subjects:
alkalitolerant
amylase
Amylases - metabolism
Geobacillus
Geobacillus stearothermophilus - enzymology
Geobacillus stearothermophilus HP 3
Hot Temperature
Hydrogen-Ion Concentration
thermostable
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study reports the production and characterisation of a novel thermostable alkaline amylase from the newly isolated Geobacillus stearothermophilus HP 3. The optimum temperature and pH for enzyme production was 55°C and 9.0, respectively. The amylase powder obtained from the culture filtrate by pre-chilled acetone treatment was stable over a wide pH range and liquefied thick starch slurries at 75°C. Ca 2+ and Co 2+ were required for the thermostability of the enzyme preparation. The present purified amylase therefore could be defined as thermostable and alkalitolerant with new properties that make the enzyme applicable for many starch processing applications for use in the food industry.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2011.583923