Antitumoural activity of viniferin-enriched extracts from Vitis vinifera L. cell cultures

The aim of this work was to evaluate the effect of stilbenes from different cultivars of Vitis vinifera on tumour proliferation. Extracts were obtained from elicited V. vinifera cell cultures and characterised by HPLC/DAD/MS. Cell growth was evaluated in four human cancer cell lines and in normal hu...

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Bibliographic Details
Published in:Natural product research 2014-01, Vol.28 (22), p.2006-2016
Main Authors: Giovannelli, L., Innocenti, M., Santamaria, A.R., Bigagli, E., Pasqua, G., Mulinacci, N.
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic
Benzofurans - isolation & purification
Benzofurans - pharmacology
breast cancer cell lines
Cell Culture Techniques
Cell Proliferation - drug effects
Chromatography, High Pressure Liquid
Chromatography, Liquid
Fibroblasts - drug effects
hepatocellular carcinoma cells
Humans
Mass Spectrometry
Plant Extracts - chemistry
Stereoisomerism
stilbenes
Stilbenes - analysis
Stilbenes - pharmacology
trans-resveratrol
viniferins
Vitis - chemistry
Vitis - genetics
Vitis vinifera
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Summary:The aim of this work was to evaluate the effect of stilbenes from different cultivars of Vitis vinifera on tumour proliferation. Extracts were obtained from elicited V. vinifera cell cultures and characterised by HPLC/DAD/MS. Cell growth was evaluated in four human cancer cell lines and in normal human fibroblasts. The cells were exposed to the extracts or to trans-resveratrol, used as reference molecule, for 48 h, at 1-10 μM concentrations of total stilbenoids. All the extracts exhibited antiproliferative activity, mediated by modulation of the cell cycle and induction of cytotoxicity in cancer but not in normal cell lines, and positively correlated with the content in dimeric stilbenoids. The Alphonse Lavallée extract was the most active, and the obtained stilbenoid fraction resulted 8-10 times more active than trans-resveratrol. Extracts from V. vinifera cell cultures could represent new sources of active stilbenoid compounds to be further assayed in in vivo studies for their antitumoural properties.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2014.924935