Simultaneous ultrasound-assisted water extraction and β-cyclodextrin encapsulation of polyphenols from Mangifera indica stem bark in counteracting TNFα-induced endothelial dysfunction

• Published in: Natural product research 2015-09, Vol.29 (17), p.1657-1663
• Main Authors: Mura, Marzia, Palmieri, Daniela, Garella, Davide, Di Stilo, Antonella, Perego, Patrizia, Cravotto, Giancarlo, Palombo, Domenico
• Format: Article
• Language: English
• Subjects: beta-Cyclodextrins; Cell Line; Cyclooxygenase 2 - metabolism; Endothelial Cells - drug effects; endothelial dysfunction; Humans; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Mangifera - chemistry; Mangifera indica; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Nitric Oxide Synthase Type III - metabolism; oestrogen receptor; p38 Mitogen-Activated Protein Kinases - metabolism; Phosphorylation; Plant Bark - chemistry; Plant Extracts - chemistry; Polyphenols - chemistry; Signal Transduction; TNFα; Tumor Necrosis Factor-alpha - pharmacology; Ultrasonics; ultrasound-assisted extraction; β-cyclodextrin
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2014.996753

This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. β-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the β-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and β-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.