Determination of phenolic compounds, in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis

• Published in: Natural product research 2020-04, Vol.34 (7), p.995-1001
• Main Authors: Leal, Ana Ediléia Barbosa Pereira, de Oliveira, Ana Paula, Santos, Raira Feitosa dos, Soares, Juliana Mikaelly Dias, Lavor, Erica Martins de, Pontes, Michelle Cruz, Lima, Julianeli Tolentino de, Santos, Alan Diego da Conceição, Tomaz, José Carlos, Oliveira, Gibson Gomes de, Neto, Fausto Carnevale, Lopes, Norberto Peporine, Rolim, Larissa Araújo, Almeida, Jackson Roberto Guedes da Silva
• Format: Article
• Language: English
• Subjects: Antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Bleaching; Carotene; Chromatography, High Pressure Liquid - methods; Equivalence; Ethanol; Flavonoids; Flavonoids - analysis; Flavonoids - chemistry; Gallic acid; High-performance liquid chromatography; Linoleic acid; Liquid chromatography; Maceration; Metabolites; Passiflora - chemistry; Passiflora cincinnata; Phenolic compounds; Phenols; Phenols - analysis; Plant extracts; Plant Extracts - chemistry; Polyphenols; Polyphenols - analysis; Quercetin; Quercetin - analysis; Scavenging; Secondary Metabolism; Secondary metabolites; Sulfonic acid; Tandem Mass Spectrometry - methods; vitexin; β-Carotene
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2018.1548445

Ethanol extracts of different parts of Passiflora cincinnata were obtained by maceration. The total phenolic and flavonoid contents were evaluated. The antioxidant activities were determined by β-carotene-linoleic acid bleaching test, 2,2-diphenyl-1-picrylhydrazil (DPPH), and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging. The crude ethanol stem extract showed the highest amount of total polyphenols (45.53 mg gallic acid equivalent/g) while the highest total flavonoid contents (1.42 mg of quercetin equivalent/g) were observed in the leaf extract. The lowest IC 50 (25.65 μg/ml) by the DPPH method was observed for the stem extract. The ABTS method showed a significant antioxidant activity for all investigated extracts. The secondary metabolite composition of ethanol extracts was assessed by HPLC-DAD-MS/MS analysis, leading to the identification of fourteen secondary metabolites in P. cincinnata extracts. These results showed the potentiality of this species as a source of phenolic compounds and antioxidants.