Genotoxicity, cytotoxicity and chemical profile from Inga laurina (Fabaceae)

This study aimed to evaluate the cytotoxicity and genotoxicity from Inga laurina leaves extracts and fractions and obtain their chemical profile. The chemical profile of the crude extract from I. laurina leaves and its fractions was investigated through 1 H NMR, RP-HPLC-PDA by co-injection with auth...

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Bibliographic Details
Published in:Natural product research 2021-02, Vol.35 (4), p.676-680
Main Authors: Falcoski, T. O. R., Lima, N. M., Navegante, G., Serafim, R. B., Sorbo, J. M., Valente, V., Santos, V. N. C., Santos, R. A., Silva, D. H. S., Soares, C. P.
Format: Article
Language:English
Subjects:
Animals
Bioassays
Biomarkers
Biotechnology
Cancer
Cell Death - drug effects
Chromatography, High Pressure Liquid
Cinnamic acid
Coinjection
Comet Assay
Coumaric acid
Cytotoxicity
Damage detection
DNA Damage
Enzyme Induction - drug effects
Fabaceae
Fabaceae - chemistry
Gallic acid
Genotoxicity
Hep G2 Cells
Hepatocellular carcinoma
High-performance liquid chromatography
Humans
Inga laurina
Liquid chromatography
Mice
Mutagens - toxicity
NAD(P)H Dehydrogenase (Quinone) - biosynthesis
NMR
Nuclear magnetic resonance
p-Coumaric acid
phenolic compounds
Phenols
Phytochemicals - analysis
Phytochemicals - pharmacology
Plant Extracts - chemistry
Plant Leaves - chemistry
Quinones
Reductases
Sulforhodamine
Toxicity
Tumor cell lines
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Summary:This study aimed to evaluate the cytotoxicity and genotoxicity from Inga laurina leaves extracts and fractions and obtain their chemical profile. The chemical profile of the crude extract from I. laurina leaves and its fractions was investigated through 1 H NMR, RP-HPLC-PDA by co-injection with authentic standards and HPLC-MS. The quinone reductase induction as a biomarker for cancer chemoprevention was evaluated in murine hepatocellular carcinoma line, whereas the cytotoxicity was evaluated by sulforhodamine B assay (SRB) using HepG2 cell line and genotoxicity was evaluated by comet assay. The phytochemical analysis of the leaves crude extract and its fractions showed the presence of 2-hydroxyethyl-dodecanoate and the phenolic compounds: gallic acid, methyl gallate, p-coumaric acid, cinnamic acid, myricetin-3-O-(2″-O-galoyl)-α-rhamnopyranoside, proanthocyanidin A-2 and myricetrin. All the fractions tested were not considered cytotoxic against the selected human cancer cell lines, they did not cause genotoxic in some concentrations damage and induced the enzyme quinone reductase.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2019.1590711