Chemical study, antioxidant activity, and genotoxicity and cytotoxicity evaluation of Ruellia angustiflora

Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical flui...

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Bibliographic Details
Published in:Natural product research 2021-12, Vol.35 (23), p.5317-5322
Main Authors: Pires, Fernanda B., Dolwitsch, Carolina B., Ugalde, Gustavo A., Menezes, Bryan B., Fontana, Marlos Eduardo Z., Rieffeld, Roberta C., Sagrillo, Michele R., Essi, Liliana, Mazutti, Marcio Antonio, da Rosa, Marcelo B., Pizzutti, Ionara Regina
Format: Article
Language:English
Subjects:
antioxidant activity
Antioxidants
Carbon dioxide
Cell viability
Chemical activity
Chemical characterization
cyto-genotoxicity
Cytotoxicity
DNA damage
Fatty acids
Flavonoids
Gas chromatography
Genotoxicity
Leukocytes (mononuclear)
Liquid chromatography
Peripheral blood mononuclear cells
Phenolic acids
Phenols
Phytosterols
Ruellia
Ruellia angustiflora
supercritical fluid extraction
Supercritical fluids
Supercritical gas extraction
Tocopherols
Toxicity
Triterpenes
Ultrasound
ultrasound-assisted extraction
Wound healing
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Summary:Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical fluid extractions, and subsequently investigate their antioxidant potential and cyto-genotoxicity. The extract obtained by ultrasound (UAE-EtOH) was characterized by ultraperformance liquid chromatography-mass spectrometer (UPLC-MS), and the extract obtained via supercritical fluid (SFE-CO 2 ) by gas chromatograph-mass spectrometer (GC-MS). The antioxidant potential was verified by the antiradical activity against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and the cyto-genotoxicity evaluation to test cell viability and DNA damage was performed in peripheral blood mononuclear cells (PBMC) cultures. The SFE-CO 2 extract presented some fatty acids, triterpenes, tetraterpenes, tocopherols and phytosterols. The UAE-EtOH extract contained phenolic acids and flavonoids, and showed the highest antioxidant capacity. Neither extract was genotoxic or cytotoxic at the tested concentrations.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2020.1753732