3.5-scFv-β-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive

A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of , affording in suc...

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Bibliographic Details
Published in:Natural product research 2022-03, Vol.36 (5), p.1317-1320
Main Authors: Simonovic, Mladen, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris
Format: Article
Language:English
Subjects:
beta-Lactamases - genetics
beta-Lactamases - metabolism
Explosive Agents
Immunoglobulin Variable Region - genetics
Immunoglobulin Variable Region - metabolism
Nitrates
Picrates
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Summary:A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of , affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 ± 2 fmol or 175 ± 6 pg/mL).[Formula: see text].
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2020.1860977