Efficient preparation of hyperoside and quercitrin from Zanthoxylum bungeanum Maxim leaves using an integrated surfactant-based aqueous two-phase system, back-extraction and adsorption separation

Zanthoxylum bungeanum leaves (ZBL) are of great medicinal value for being rich in hyperoside and quercitrin. In this study, a novel, efficient and economical continuous process was established. First, aqueous two-phase system (ATPS) composed of Triton X-100/(NH 4 ) 2 SO 4 was employed to enrich hype...

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Bibliographic Details
Published in:Natural product research 2024-09, Vol.38 (18), p.3188-3200
Main Authors: Han, Nuan, Chen, Xiaoqi, Wang, Hong, Wang, Nana, Nian, Miaoxiang, Wang, Dongmei
Format: Article
Language:English
Subjects:
Adsorption
Ammonium sulfate
aqueous two-phase system
back-extraction
Binary systems
Chromatography, High Pressure Liquid - methods
Dichloromethane
Flavonoids
Flavonoids - chemistry
Flavonoids - isolation & purification
hyperoside
Industrial production
macroporous resin
Micelles
Plant Extracts - chemistry
Plant Leaves - chemistry
Quercetin - analogs & derivatives
Quercetin - chemistry
Quercetin - isolation & purification
quercitrin
Surface-Active Agents - chemistry
Water reuse
Zanthoxylum - chemistry
Zanthoxylum bungeanum
Zanthoxylum bungeanum leaves
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Summary:Zanthoxylum bungeanum leaves (ZBL) are of great medicinal value for being rich in hyperoside and quercitrin. In this study, a novel, efficient and economical continuous process was established. First, aqueous two-phase system (ATPS) composed of Triton X-100/(NH 4 ) 2 SO 4 was employed to enrich hyperoside and quercitrin from ZBL extracts and the recoveries reached 98.53% and 99.12%. Then back-extraction with dichloromethane-water system was adopted to separate hyperoside and quercitrin from Triton X-100 micelles which were recycled and the recoveries reached 86.58% and 85.19%. Finally, S-8 macroporous resin was used for removing the salt introduced in ATPS and the final recoveries reached 82.38% and 81.81%, much higher than the total flavonoids recovery as 69.08%. Furthermore, scale-up experiment certified that the continuous process was feasible for industrial production. Efficiently and economically, this method achieved a great breakthrough in purity and provided a novel reference for further purification and phase-forming component recycle.
ISSN:1478-6419
1478-6427
1478-6427
DOI:10.1080/14786419.2023.2219822