In vitro spectroscopic investigation of groove binding interaction of Fe 3 O 4 @CaAl-LDH@L-Dopa with calf thymus DNA

The principal goal of this study is to evaluate the interaction of Fe O @CaAl-LDH@L-Dopa and Fe O @CaAl-LDH nanoparticles with calf thymus DNA. The magnetic nanoparticles were previously prepared by a chemical co-precipitation method, and the surface of the Fe O nanoparticles was coated with CaAl la...

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Bibliographic Details
Published in:Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2020-07, Vol.39 (7), p.1020-1035
Main Authors: Shahabadi, Nahid, Razlansari, Mahtab
Format: Article
Language:English
Subjects:
Aluminum Oxide - chemistry
Animals
Binding Sites
Calcium Chloride - chemistry
Cattle
Circular Dichroism
DNA - chemistry
Ferric Compounds - chemistry
Levodopa - chemistry
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Viscosity
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Summary:The principal goal of this study is to evaluate the interaction of Fe O @CaAl-LDH@L-Dopa and Fe O @CaAl-LDH nanoparticles with calf thymus DNA. The magnetic nanoparticles were previously prepared by a chemical co-precipitation method, and the surface of the Fe O nanoparticles was coated with CaAl layered double hydroxides. The antiparkinsonian drug "L-Dopa" was carried by this core-shell nanostructure to achieve the drug delivery system with suitable properties for biological applications. Also, the interaction of Fe O @CaAl-LDH@L-Dopa and Fe O @CaAl-LDH nanoparticles with CT-DNA was studied using, UV-Visible spectroscopy, viscosity, circular dichroism (CD), and fluorescence spectroscopy techniques. The results of investigations demonstrated that Fe O @CaAl-LDH@L-Dopa and Fe O @CaAl-LDH nanoparticles have interacted via minor groove binding and intercalated to CT-DNA, respectively.
ISSN:1525-7770
1532-2335
DOI:10.1080/15257770.2020.1740929