Recognition of 2′-Deoxyisoinosine Triphosphate by the Klenow Fragment of DNA Polymerase I

The capability of the Klenow fragment deficient in 3′→5′ exonuclease activity (KFexo − ) to incorporate 2′-deoxyisoinosine (disol) opposite canonical bases and 5-methyl-2-deoxyisocytidine (d 5Me isoC) was examined. The triphosphate of disol is incorporated opposite d 5Me isoC designed as its pairing...

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Bibliographic Details
Published in:Nucleosides & nucleotides 1999-03, Vol.18 (3), p.403-410
Main Authors: Beaussire, Jean-Jacques, Pochet, Sylvie
Format: Article
Language:English
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Summary:The capability of the Klenow fragment deficient in 3′→5′ exonuclease activity (KFexo − ) to incorporate 2′-deoxyisoinosine (disol) opposite canonical bases and 5-methyl-2-deoxyisocytidine (d 5Me isoC) was examined. The triphosphate of disol is incorporated opposite d 5Me isoC designed as its pairing partner. It is also incorporated opposite T and G according to a pairing scheme involving its minor tautomeric form (the O 2 H tautomer).
ISSN:0732-8311
DOI:10.1080/15257779908043085