PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION

This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-o...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2012-04, Vol.33 (2), p.115-127
Main Authors: Dykman, Lev A., Staroverov, Sergei A., Guliy, Olga I., Ignatov, Oleg V., Fomin, Alexander S., Vidyasheva, Irina V., Karavaeva, Olga A., Bunin, Viktor D., Burygin, Gennady L.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - chemistry
Antibodies, Bacterial - immunology
Antigens, Bacterial - immunology
Antigens, Surface - immunology
Azospirillum brasilense - classification
Azospirillum brasilense - immunology
Azospirillum brasilense - isolation & purification
Azospirillum brasilense Sp245
Combinatorial Chemistry Techniques
Electrochemistry
electron microscopy
Escherichia coli - immunology
flagellin
Flagellin - analysis
Flagellin - immunology
Immunoblotting
lipopolysaccharide
Lipopolysaccharides - analysis
Lipopolysaccharides - immunology
Microscopy, Electron, Transmission
orientational spectrum
phage-displayed miniantibodies
Sheep
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Summary:This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2011.603775