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PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION
This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-o...
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| Published in: | Journal of immunoassay & immunochemistry 2012-04, Vol.33 (2), p.115-127 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c362t-4b82b5f6b6ce9df3c9c1995ca83d654abacc11e6ad4ecaa163d222358e20154d3 |
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| container_end_page | 127 |
| container_issue | 2 |
| container_start_page | 115 |
| container_title | Journal of immunoassay & immunochemistry |
| container_volume | 33 |
| creator | Dykman, Lev A. Staroverov, Sergei A. Guliy, Olga I. Ignatov, Oleg V. Fomin, Alexander S. Vidyasheva, Irina V. Karavaeva, Olga A. Bunin, Viktor D. Burygin, Gennady L. |
| description | This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions. |
| doi_str_mv | 10.1080/15321819.2011.603775 |
| format | article |
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The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.</description><identifier>ISSN: 1532-1819</identifier><identifier>EISSN: 1532-4230</identifier><identifier>DOI: 10.1080/15321819.2011.603775</identifier><identifier>PMID: 22471602</identifier><language>eng</language><publisher>England: Taylor & Francis Group</publisher><subject>Animals ; Antibodies, Bacterial - chemistry ; Antibodies, Bacterial - immunology ; Antigens, Bacterial - immunology ; Antigens, Surface - immunology ; Azospirillum brasilense - classification ; Azospirillum brasilense - immunology ; Azospirillum brasilense - isolation & purification ; Azospirillum brasilense Sp245 ; Combinatorial Chemistry Techniques ; Electrochemistry ; electron microscopy ; Escherichia coli - immunology ; flagellin ; Flagellin - analysis ; Flagellin - immunology ; Immunoblotting ; lipopolysaccharide ; Lipopolysaccharides - analysis ; Lipopolysaccharides - immunology ; Microscopy, Electron, Transmission ; orientational spectrum ; phage-displayed miniantibodies ; Sheep</subject><ispartof>Journal of immunoassay & immunochemistry, 2012-04, Vol.33 (2), p.115-127</ispartof><rights>Copyright Taylor & Francis Group, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-4b82b5f6b6ce9df3c9c1995ca83d654abacc11e6ad4ecaa163d222358e20154d3</citedby><cites>FETCH-LOGICAL-c362t-4b82b5f6b6ce9df3c9c1995ca83d654abacc11e6ad4ecaa163d222358e20154d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22471602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dykman, Lev A.</creatorcontrib><creatorcontrib>Staroverov, Sergei A.</creatorcontrib><creatorcontrib>Guliy, Olga I.</creatorcontrib><creatorcontrib>Ignatov, Oleg V.</creatorcontrib><creatorcontrib>Fomin, Alexander S.</creatorcontrib><creatorcontrib>Vidyasheva, Irina V.</creatorcontrib><creatorcontrib>Karavaeva, Olga A.</creatorcontrib><creatorcontrib>Bunin, Viktor D.</creatorcontrib><creatorcontrib>Burygin, Gennady L.</creatorcontrib><title>PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION</title><title>Journal of immunoassay & immunochemistry</title><addtitle>J Immunoassay Immunochem</addtitle><description>This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.</description><subject>Animals</subject><subject>Antibodies, Bacterial - chemistry</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antigens, Surface - immunology</subject><subject>Azospirillum brasilense - classification</subject><subject>Azospirillum brasilense - immunology</subject><subject>Azospirillum brasilense - isolation & purification</subject><subject>Azospirillum brasilense Sp245</subject><subject>Combinatorial Chemistry Techniques</subject><subject>Electrochemistry</subject><subject>electron microscopy</subject><subject>Escherichia coli - immunology</subject><subject>flagellin</subject><subject>Flagellin - analysis</subject><subject>Flagellin - immunology</subject><subject>Immunoblotting</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides - analysis</subject><subject>Lipopolysaccharides - immunology</subject><subject>Microscopy, Electron, Transmission</subject><subject>orientational spectrum</subject><subject>phage-displayed miniantibodies</subject><subject>Sheep</subject><issn>1532-1819</issn><issn>1532-4230</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kF1LwzAUhoMofv8Dkdx5tZmPJmuvpHaZFuYqbXcd0jSFSrvOZEXmr7dl00uvzuHwvOeFB4A7jKYY-egRM0qwj4MpQRhPOaKzGTsBl-N54hGKTo_7yFyAK-c-EMLBDAXn4IIQb4Y5Ipegf0_Fe5iGeZysYLKAb_EqDld5_JzMY5HBPIHhd-e2ta2bpm9hYZWrG7NxBmZb4jGYrdNFGAk4Zl7EKhuWOcxfRZzCdSbgIknhcxjlIo3DJZyLXERj0w04q1TjzO1xXoP1QuTR62SZvMRRuJxoyslu4hU-KVjFC65NUFZUBxoHAdPKpyVnniqU1hgbrkrPaKUwpyUhhDLfDE6YV9Jr8HD4u7XdZ2_cTra106Zp1MZ0vZMB9zFiBNOB9A6ktp1z1lRya-tW2b3ESI6-5a9vOfqWB99D7P5Y0BetKf9Cv4IH4OkA1Juqs6366mxTyp3aN52trNro2kn6b8UPh4aHvA</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Dykman, Lev A.</creator><creator>Staroverov, Sergei A.</creator><creator>Guliy, Olga I.</creator><creator>Ignatov, Oleg V.</creator><creator>Fomin, Alexander S.</creator><creator>Vidyasheva, Irina V.</creator><creator>Karavaeva, Olga A.</creator><creator>Bunin, Viktor D.</creator><creator>Burygin, Gennady L.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION</title><author>Dykman, Lev A. ; Staroverov, Sergei A. ; Guliy, Olga I. ; Ignatov, Oleg V. ; Fomin, Alexander S. ; Vidyasheva, Irina V. ; Karavaeva, Olga A. ; Bunin, Viktor D. ; Burygin, Gennady L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-4b82b5f6b6ce9df3c9c1995ca83d654abacc11e6ad4ecaa163d222358e20154d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - chemistry</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antigens, Bacterial - immunology</topic><topic>Antigens, Surface - immunology</topic><topic>Azospirillum brasilense - classification</topic><topic>Azospirillum brasilense - immunology</topic><topic>Azospirillum brasilense - isolation & purification</topic><topic>Azospirillum brasilense Sp245</topic><topic>Combinatorial Chemistry Techniques</topic><topic>Electrochemistry</topic><topic>electron microscopy</topic><topic>Escherichia coli - immunology</topic><topic>flagellin</topic><topic>Flagellin - analysis</topic><topic>Flagellin - immunology</topic><topic>Immunoblotting</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides - analysis</topic><topic>Lipopolysaccharides - immunology</topic><topic>Microscopy, Electron, Transmission</topic><topic>orientational spectrum</topic><topic>phage-displayed miniantibodies</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dykman, Lev A.</creatorcontrib><creatorcontrib>Staroverov, Sergei A.</creatorcontrib><creatorcontrib>Guliy, Olga I.</creatorcontrib><creatorcontrib>Ignatov, Oleg V.</creatorcontrib><creatorcontrib>Fomin, Alexander S.</creatorcontrib><creatorcontrib>Vidyasheva, Irina V.</creatorcontrib><creatorcontrib>Karavaeva, Olga A.</creatorcontrib><creatorcontrib>Bunin, Viktor D.</creatorcontrib><creatorcontrib>Burygin, Gennady L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunoassay & immunochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dykman, Lev A.</au><au>Staroverov, Sergei A.</au><au>Guliy, Olga I.</au><au>Ignatov, Oleg V.</au><au>Fomin, Alexander S.</au><au>Vidyasheva, Irina V.</au><au>Karavaeva, Olga A.</au><au>Bunin, Viktor D.</au><au>Burygin, Gennady L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION</atitle><jtitle>Journal of immunoassay & immunochemistry</jtitle><addtitle>J Immunoassay Immunochem</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>33</volume><issue>2</issue><spage>115</spage><epage>127</epage><pages>115-127</pages><issn>1532-1819</issn><eissn>1532-4230</eissn><abstract>This article reports the first preparation of miniantibodies to Azospirillum brasilense Sp245 surface antigens by using a combinatorial phage library of sheep antibodies. The prepared phage antibodies were used for the first time for lipopolysaccharide and flagellin detection by dot assay, electro-optical analysis of cell suspensions, and transmission electron microscopy. Interaction of A. brasilense Sp245 with antilipopolysaccharide and antiflagellin phage-displayed miniantibodies caused the magnitude of the electro-optical signal to change considerably. The electro-optical results were in good agreement with the electron microscopic data. This is the first reported possibility of employing phage-displayed miniantibodies in bacterial detection aided by electro-optical analysis of cell suspensions.</abstract><cop>England</cop><pub>Taylor & Francis Group</pub><pmid>22471602</pmid><doi>10.1080/15321819.2011.603775</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of immunoassay & immunochemistry, 2012-04, Vol.33 (2), p.115-127 |
| issn | 1532-1819 1532-4230 |
| language | eng |
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| source | Taylor and Francis Science and Technology Collection |
| subjects | Animals Antibodies, Bacterial - chemistry Antibodies, Bacterial - immunology Antigens, Bacterial - immunology Antigens, Surface - immunology Azospirillum brasilense - classification Azospirillum brasilense - immunology Azospirillum brasilense - isolation & purification Azospirillum brasilense Sp245 Combinatorial Chemistry Techniques Electrochemistry electron microscopy Escherichia coli - immunology flagellin Flagellin - analysis Flagellin - immunology Immunoblotting lipopolysaccharide Lipopolysaccharides - analysis Lipopolysaccharides - immunology Microscopy, Electron, Transmission orientational spectrum phage-displayed miniantibodies Sheep |
| title | PREPARATION OF MINIANTIBODIES TO Azospirillum brasilense Sp245 SURFACE ANTIGENS AND THEIR USE FOR BACTERIAL DETECTION |
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