MONOCLONAL ANTIBODIES REQUIRING COATING BUFFER WITH LOW PH FOR EFFICIENT ANTIGEN CAPTURE IN SANDWICH ELISA: THE RARITIES OR PRACTICALLY IMPORTANT PHENOMENA?

This article reexamines some opinions concerning pH requirements for optimal immobilization of monoclonal antibodies (mAbs) by passive adsorption in antigen capture ELISA. It was discovered that substitution of "classical" sodium phosphate (pH 7.5) and carbonate (pH 9.5) coating solutions...

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Published in:Journal of immunoassay & immunochemistry 2013-10, Vol.34 (4), p.414-437
Main Authors: Dmitriev, Alexander D., Tarakanova, Julia N., Yakovleva, Dinora A., Dmitriev, Dmitriy A., Phartooshnaya, Olga V., Kolyaskina, Galina I., Massino, Yulia S., Borisova, Olga V., Segal, Olga L., Smirnova, Maria B., Ulanova, Tatiana I., Lavrov, Viacheslav F.
Format: Article
Language:English
Subjects:
Adsorption
analytical sensitivity
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - immunology
antibody adsorption
Antigen-Antibody Reactions
Antigens - isolation & purification
Buffers
coating buffer pH
Enzyme-Linked Immunosorbent Assay - instrumentation
Enzyme-Linked Immunosorbent Assay - methods
Hepatitis B Surface Antigens - immunology
Humans
Hydrogen-Ion Concentration
Immunoassay - methods
immunoassay optimization
monoclonal antibodies
Rabbits
sandwich ELISA
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cited_by cdi_FETCH-LOGICAL-c396t-b8f35d1b92d21887060c2f6321ef17e9a1a90ad5dccbbbdbf48d649ebd6731d53
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container_issue 4
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container_title Journal of immunoassay & immunochemistry
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creator Dmitriev, Alexander D.
Tarakanova, Julia N.
Yakovleva, Dinora A.
Dmitriev, Dmitriy A.
Phartooshnaya, Olga V.
Kolyaskina, Galina I.
Massino, Yulia S.
Borisova, Olga V.
Segal, Olga L.
Smirnova, Maria B.
Ulanova, Tatiana I.
Lavrov, Viacheslav F.
description This article reexamines some opinions concerning pH requirements for optimal immobilization of monoclonal antibodies (mAbs) by passive adsorption in antigen capture ELISA. It was discovered that substitution of "classical" sodium phosphate (pH 7.5) and carbonate (pH 9.5) coating solutions by acid (pH 2.8) buffers maximized antigen capture 4 out of 10 different tested anti-HBsAg mAbs, resulting in a 1.5-2.5 increase of binding curve coefficients. By measuring both mAbs amounts and functionality, the enhancement effect was attributed to the better preservation of solid phase antibodies activity.
doi_str_mv 10.1080/15321819.2013.764894
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source Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list)
subjects Adsorption
analytical sensitivity
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - immunology
antibody adsorption
Antigen-Antibody Reactions
Antigens - isolation & purification
Buffers
coating buffer pH
Enzyme-Linked Immunosorbent Assay - instrumentation
Enzyme-Linked Immunosorbent Assay - methods
Hepatitis B Surface Antigens - immunology
Humans
Hydrogen-Ion Concentration
Immunoassay - methods
immunoassay optimization
monoclonal antibodies
Rabbits
sandwich ELISA
title MONOCLONAL ANTIBODIES REQUIRING COATING BUFFER WITH LOW PH FOR EFFICIENT ANTIGEN CAPTURE IN SANDWICH ELISA: THE RARITIES OR PRACTICALLY IMPORTANT PHENOMENA?
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