Human Hepatocytes Isolated from Explanted Livers: A Powerful Tool to Understand End-stage Liver Disease and Drug Screening

The use of primary human hepatocytes has been hampered by limited availability of adequate numbers of fresh and viable cells due to the ongoing shortage of liver donors. Thus, there is no surplus of healthy organs from which freshly isolated cells can be prepared when needed. However, primary hepato...

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Bibliographic Details
Published in:Organogenesis 2021-10, Vol.17 (3-4), p.117-125
Main Authors: Faccioli, Lanuza A. P., Kocas-Kilicarslan, Zehra N., Diaz-Aragon, Ricardo, Motomura, Takashi, Amirneni, Sriram, Malizio, Michelle R., Coard, Michael C., Frau, Carla, Haep, Nils, Florentino, Rodrigo M., Ostrowska, Alina
Format: Article
Language:English
Subjects:
cryopreservation
diseased liver
Drug Evaluation, Preclinical
drug screening
End Stage Liver Disease - metabolism
End Stage Liver Disease - surgery
Hepatocyte isolation
Hepatocytes - metabolism
Humans
Liver
Liver Transplantation
Review
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Summary:The use of primary human hepatocytes has been hampered by limited availability of adequate numbers of fresh and viable cells due to the ongoing shortage of liver donors. Thus, there is no surplus of healthy organs from which freshly isolated cells can be prepared when needed. However, primary hepatocytes can be successfully isolated from explanted liver specimens obtained from patients receiving orthotopic liver transplantation for decompensated liver cirrhosis or for metabolic liver disease without end-stage liver disease and are a valuable resource for the pharmaceutical industry research. This review focuses on the isolation, characterization and cryopreservation of hepatocytes derived from therapeutically resected livers with various hepatic diseases.
ISSN:1547-6278
1555-8592
DOI:10.1080/15476278.2021.1992216