LARP1 isoform expression in human cancer cell lines

LARP1 is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. From published in vitro studies, there is disparity over which of two different LARP1 protein isoforms (termed the long LI-LARP1 and short SI-LARP1) is the canonical. Here, after conducting a series...

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Bibliographic Details
Published in:RNA biology 2021-02, Vol.18 (2), p.237-247
Main Authors: Schwenzer, Hagen, Abdel Mouti, Mai, Neubert, Pia, Morris, Josephine, Stockton, Joanne, Bonham, Sarah, Fellermeyer, Martin, Chettle, James, Fischer, Roman, Beggs, Andrew D., Blagden, Sarah P.
Format: Article
Language:English
Subjects:
alternative protein isoforms
Alternative Splicing
Amino Acid Sequence
Autoantigens - chemistry
Autoantigens - genetics
Autoantigens - metabolism
cancer
Cell Line, Tumor
DNA Methylation
Gene Expression Regulation, Neoplastic
Gene Silencing
Humans
LARP1
Multigene Family
Organ Specificity
Promoter Regions, Genetic
Protein Binding
Protein Isoforms - genetics
Protein Isoforms - metabolism
Research Paper
Ribonucleoproteins - chemistry
Ribonucleoproteins - genetics
Ribonucleoproteins - metabolism
RNA - genetics
RNA - metabolism
RNA Binding Proteins
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
SS-B Antigen
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Summary:LARP1 is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. From published in vitro studies, there is disparity over which of two different LARP1 protein isoforms (termed the long LI-LARP1 and short SI-LARP1) is the canonical. Here, after conducting a series of biochemical and cellular assays, we conclude that LI-LARP1 (NM_033551.3 > NP_056130.2) is the dominantly expressed form. We observe that SI-LARP1 (NM_015315.5> NP_056130.2) is epigenetically repressed and that this repression is evolutionarily conserved in all but a small subclade of mammalian species. As with other LARP family members, there are multiple potential LARP1 mRNA isoforms that appear to be censored within the nucleus. The capacity of the cell to modulate splicing and expression of these apparently 'redundant' mRNAs hints at contextually specific mechanisms of LARP1 expression.
ISSN:1547-6286
1555-8584
DOI:10.1080/15476286.2020.1744320