A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA Trp to decode the UGA codon in Trypanosoma brucei

In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA ) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic tra...

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Bibliographic Details
Published in:RNA biology 2021-10, Vol.18 (sup1), p.278-286
Main Authors: Paris, Zdeněk, Svobodová, Michaela, Kachale, Ambar, Horáková, Eva, Nenarokova, Anna, Lukeš, Julius
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Codon, Terminator
Cytidine - chemistry
Cytidine - genetics
Cytidine Deaminase - genetics
Cytidine Deaminase - metabolism
Mitochondria - enzymology
Mitochondria - genetics
Nucleic Acid Conformation
RNA, Mitochondrial - analysis
RNA, Mitochondrial - genetics
RNA, Protozoan - analysis
RNA, Protozoan - genetics
RNA, Transfer, Trp
Sequence Homology
Trypanosoma brucei brucei - genetics
Trypanosoma brucei brucei - growth & development
Trypanosoma brucei brucei - metabolism
Uridine - chemistry
Uridine - genetics
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Summary:In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA ) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA , this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in . The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.
ISSN:1547-6286
1555-8584
DOI:10.1080/15476286.2021.1940445