Endogenous ZAP affects Zika virus RNA interactome

• Published in: RNA biology 2024-12, Vol.21 (1), p.849-858
• Main Authors: Sabir, Ahmad Jawad, Le, Nguyen Phuong Khanh, Singh, Prince Pal, Karniychuk, Uladzimir
• Format: Article
• Language: English
• Subjects: antiviral agents; antiviral properties; antiviral proteins; Brief Communication; Flavivirus; HEK293 Cells; Host-Pathogen Interactions - genetics; Humans; interactome; Japanese encephalitis virus; Mass Spectrometry; Protein Binding; protein-protein interactions; Rapid Communication; RNA; RNA helicase; RNA, Viral - genetics; RNA, Viral - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; West Nile virus; ZAP; Zika virus; Zika Virus - genetics; Zika Virus - metabolism; Zika Virus - physiology; Zika Virus Infection - metabolism; Zika Virus Infection - virology; zinc finger antiviral protein; zinc finger motif
• ISSN: 1547-6286; 1555-8584; 1555-8584
• DOI: 10.1080/15476286.2024.2388911

One of the most recent advances in the analysis of viral RNA-cellular protein interactions is the Comprehensive Identification of RNA-binding Proteins by Mass Spectrometry (ChIRP-MS). Here, we used ChIRP-MS in mock-infected and Zika-infected wild-type cells and cells knockout for the zinc finger CCCH-type antiviral protein 1 (ZAP). We characterized 'ZAP-independent' and 'ZAP-dependent' cellular protein interactomes associated with flavivirus RNA and found that ZAP affects cellular proteins associated with Zika virus RNA. The ZAP-dependent interactome identified with ChIRP-MS provides potential ZAP co-factors for antiviral activity against Zika virus and possibly other viruses. Identifying the full spectrum of ZAP co-factors and mechanisms of how they act will be critical to understanding the ZAP antiviral system and may contribute to the development of antivirals.