Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identi...

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Bibliographic Details
Published in:Mycologia 2002-05, Vol.94 (3), p.450-460
Main Authors: Ceresini, Paulo C., Shew, H. David, Vilgalys, Rytas J., Rosewich, U. Liane, Cubeta, Marc A.
Format: Article
Language:English
Subjects:
Alleles
DNA
Genetic diversity
Genetic loci
genetic markers
genetic variation
Genetics/Molecular Biology
genotype
Genotypes
heterozygosity
host plants
linkage disequilibrium
loci
migration
molecular sequence data
nucleotide sequences
Phytopathology
plant pathogenic fungi
polymerase chain reaction
Population genetics
Population geography
Population structure
population subdivision
potatoes
restriction fragment length polymorphism
Rhizoctonia
soil fungi
Solanum tuberosum
Thanatephorus cucumeris
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Summary:A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.
ISSN:0027-5514
1557-2536
DOI:10.1080/15572536.2003.11833210