The protective effects of chitosan oligosaccharide (COS) on cadmium-induced neurotoxicity in Wistar rats

• Published in: Archives of environmental & occupational health 2022-09, Vol.77 (9), p.755-763
• Main Authors: Kısadere, İhsan, Karaman, Musa, Aydın, Mehmet Faruk, Donmez, Nurcan, Usta, Mustafa
• Format: Article
• Language: English
• Subjects: Antioxidants; Cadmium; Cadmium chloride; Cerebral cortex; Chitosan; chitosan oligosaccharide; cytokine; Cytokines; Hippocampus; Immunoreactivity; Interleukin 10; Interleukin 6; Neurotoxicity; Oligosaccharides; rat; Rodents; Synaptophysin; Tumor necrosis factor-α
• ISSN: 1933-8244; 2154-4700
• DOI: 10.1080/19338244.2021.2008852

The aim of the study was to investigate the influence of chitosan oligosaccharide (COS) on some antioxidant and cytokine levels in the rat hippocampus as well as synaptophysin (SYP) immunoreactivity in the cerebral cortex of the cadmium (Cd) exposed rats. Thirty-two male albino Wistar rats were divided randomly into four equal groups as control (C; n = 8), Cd (n = 8), COS (n = 8), and Cd + COS (CdCOS; n = 8). The rats in the Cd and CdCOS groups received Cd chloride (CdCl 2 ) (2 mg/kg/d) orally by gastric gavage three times a week for 4 weeks. Besides, COS (200 mg/kg/d) was administered to COS and CdCOS groups five times a week for 4 weeks. Then, they were decapitated and hippocampal/cerebral cortex tissue samples were taken for measurement of GSH levels, CAT and SOD activities, MDA values, TNF-α, IL-6, and IL-10 levels as well as SYP immunoreactivity. Although tissue GSH levels were determined the lowest in the Cd group, these values were attenuated with COS treatment in the CdCOS group (p