HPLC-DAD analysis of florfenicol and thiamphenicol in medicated feedingstuffs

A simple and reliable method using liquid chromatography with diode array detector was developed for the simultaneous determination of florfenicol and thiamphenicol in medicated feed. The analytes were extracted from the minced feed with methanol and ethyl acetate (1:1, v/v). Next, the extract was f...

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Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2019-08, Vol.36 (8), p.1184-1190
Main Authors: Patyra, Ewelina, Kwiatek, Krzysztof
Format: Article
Language:English
Subjects:
Acetic acid
Animal feed
d-SPE
DAD
Ethyl acetate
Farms
Florfenicol
High performance liquid chromatography
HPLC
in-house validation method
Liquid chromatography
Magnesium
Magnesium sulfate
medicated feed
Medicated feeds
Poultry farming
Reproducibility
Solid phases
Sorbents
Thiamphenicol
Water purification
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Summary:A simple and reliable method using liquid chromatography with diode array detector was developed for the simultaneous determination of florfenicol and thiamphenicol in medicated feed. The analytes were extracted from the minced feed with methanol and ethyl acetate (1:1, v/v). Next, the extract was further cleaned up by dispersive solid phase extraction using anhydrous magnesium sulfate, PSA and C18 sorbents. Finally, 1 mL of extract was evaporated, the residue resuspended in Milli-Q water, and filtered. The method was validated in-house at medicated levels, in the concentration range 10-300 µg/mL (50-1500 mg/kg). Values of
ISSN:1944-0049
1944-0057
DOI:10.1080/19440049.2019.1619943