Assessment of milk quality using novel mutations of B2M gene in bovine DNA from milk

This study investigated the expression of novel mutations of the β2-microglobulin (B2M) gene in bovine genomic DNA isolated from milk-borne leukocytes and tested the association of these mutations with milk quality traits in Holstein cows. DNA was extracted using a novel protocol allowing for the am...

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Bibliographic Details
Published in:CYTA: journal of food 2018-01, Vol.16 (1), p.281-286
Main Authors: Liu, Yongfeng, Liao, Jing, Ku, Ting, Li, Xiaoling, Sheppard, Allan Michael
Format: Article
Language:English
Subjects:
ADN bovino
asociación
association
B2M gene
Bovine DNA
calidad de la leche
Deoxyribonucleic acid
DNA
DNA sequencing
gen B2M gene
Gene expression
Leukocytes
Milk
milk quality
mutaciones
Mutation
mutations
Primers
Proteins
Quality assessment
β2 Microglobulin
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Summary:This study investigated the expression of novel mutations of the β2-microglobulin (B2M) gene in bovine genomic DNA isolated from milk-borne leukocytes and tested the association of these mutations with milk quality traits in Holstein cows. DNA was extracted using a novel protocol allowing for the amplification of long fragments of DNA isolated from small amounts of unprocessed milk samples. Genomic mutations at the B2M locus were explored by DNA sequencing using specific primers and a cluster of five identified within a 729-bp region of intron 3. Specifically, mutations at 7008(G>T), 7202(A>G), 7432(G>T) and 7437(G>C) were all found to be significantly associated with milk protein percentage (P A) was significantly associated with somatic cell count (P 
ISSN:1947-6337
1947-6345
DOI:10.1080/19476337.2017.1394367