Normalization of short-chain fatty acid concentration by bacterial count of stool samples improves discrimination between eubiotic and dysbiotic gut microbiota caused by Clostridioides difficile infection

Short-chain fatty acids (SCFAs) represent a cornerstone of gut health, serving as critical mediators of immune modulation and overall host homeostasis. Patients with dysbiosis caused by Clostridioides difficile infection (CDI) typically exhibit lower SCFAs levels compared to healthy stool donors and...

Full description

Saved in:
Bibliographic Details
Published in:Gut microbes 2024-12, Vol.16 (1), p.2415488
Main Authors: Sayol-Altarriba, Anna, Aira, Andrea, Villasante, Anna, Albarracín, Rosa, Faneca, Joana, Casals, Gregori, Villanueva-Cañas, José Luis, Casals-Pascual, Climent
Format: Article
Language:English
Subjects:
Adult
Aged
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Bacterial Load
Butyrates - analysis
Butyrates - metabolism
Clostridioides difficile
Clostridioides difficile - metabolism
Clostridium Infections - microbiology
dysbiosis
Dysbiosis - microbiology
Fatty Acids, Volatile - analysis
Fatty Acids, Volatile - metabolism
fecal microbiota transplantation (FMT)
Feces - chemistry
Feces - microbiology
Female
Gastrointestinal Microbiome
gut
Humans
intestinal microbiota
Male
Middle Aged
Research Paper
Short-chain fatty acids (SCFAs)
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Short-chain fatty acids (SCFAs) represent a cornerstone of gut health, serving as critical mediators of immune modulation and overall host homeostasis. Patients with dysbiosis caused by Clostridioides difficile infection (CDI) typically exhibit lower SCFAs levels compared to healthy stool donors and, thus, the concentration of SCFAs has been proposed as a proxy marker of a healthy microbiota. However, there is no consistency in the methods used to quantify SCFAs in stool samples and usually, the results are normalized by the weight of the stool samples, which does not address differences in water and fiber content and ignores bacterial counts in the sample (the main component of stool that contributes to the composition of these metabolites in the sample). Here, we show that normalized SCFAs concentrations by the bacterial count improve discrimination between healthy and dysbiotic samples (patients with CDI), particularly when using acetate and propionate levels. After normalization, butyrate is the metabolite that best discriminates eubiotic and dysbiotic samples according to the area under the receiver operating characteristic (ROC) curve (AUC-ROC = 0.860, [95% CI: 0.786-0.934], p 
ISSN:1949-0976
1949-0984
1949-0984
DOI:10.1080/19490976.2024.2415488