Expression of thermostable β-xylosidase in Escherichia coli for use in saccharification of plant biomass

• Published in: Bioengineered 2017-09, Vol.8 (5), p.665-669
• Main Authors: Aftab, Muhammad N., Zafar, Asma, Awan, Ali R.
• Format: Article
• Language: English
• Subjects: Addendum; Bacillus licheniformis - enzymology; Bacillus licheniformis - genetics; E. coli; Enzyme Activation; Enzyme Stability; Escherichia coli - genetics; Escherichia coli - metabolism; Ethanol - isolation & purification; Ethanol - metabolism; expression; Genetic Enhancement - methods; plant biomass; Plant Components, Aerial - microbiology; Protein Engineering - methods; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; saccharification; Temperature; xylosidase; Xylosidases - genetics; Xylosidases - metabolism
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2016.1267884

The present work is aimed to evaluate the saccharification potential of a thermostable β-xylosidase cloned from Bacillus licheniformis into Escherichia coli for production of bioethanol from plant biomass. Recombinant β-xylosidase enzyme possesses the ability of bioconversion of plant biomass like wheat straw, rice straw and sugarcane bagass. By using this approach, plant biomass that mainly constitute cellulose can be converted to reducing sugars that could then be easily converted to bioethanol by simple fermentation process. The production of bioethanol will help to overcome energy requirements due to depleting fossil fuels and will also help to protect environment by reducing greenhouse gas emission. In the end, future directions are briefly mentioned that can be utilized to reduce the cost and increase the yield of biofuels.