MicroRNA miR-126 attenuates brain injury in septic rats via NF-κB signaling pathway

• Published in: Bioengineered 2021-01, Vol.12 (1), p.2639-2648
• Main Authors: Nong, Anna, Li, Qingfeng, Huang, Zhijing, Xu, Yunan, He, Kebin, Jia, Yuying, Cen, Zhenyi, Liao, Lianghua, Huang, Yueyan
• Format: Article
• Language: English
• Subjects: Animals; blood-brain barrier; Blood-Brain Barrier - metabolism; Brain Diseases - metabolism; Brain Diseases - pathology; brain injury; Male; MicroRNAs - genetics; MicroRNAs - metabolism; miR-126; NF-kappa B - genetics; NF-kappa B - metabolism; nf-κB signaling pathway; Rats; Rats, Sprague-Dawley; Research Paper; Sepsis; Sepsis - metabolism; Sepsis - pathology; Signal Transduction - genetics
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2021.1937905

The purpose of this study was to investigate the impact and mechanism of microRNA miR-126 on brain injury induced by blood-brain barrier (BBB) damage in septic rats. We used cecal ligation and perforation (CLP) to create a rat model of sepsis. The experimental rats were randomly divided into Control group, CLP group, CLP + miR-NC group, CLP + miR-126 group and CLP + miR-126 + NF-κB pathway agonist (PMA) group. MiR-126 expressed in the brain tissue of CLP rats was down-regulated by qRT-PCR. Upregulation of miR-126 in CLP rats could improve brain injury and BBB marker protein level, reduce brain water content, Evans blue extravasation, inflammation, and excessive oxidative stress. This could also result in an inhibition of NF-κB signaling pathway activity. In conclusion, miR-126 overexpression can prevent brain injury caused by BBB damage via the inhibition of NF-κB signaling pathway activity.