AMPEROMETRIC MICRO-BIOSENSOR ARRAY FOR CHOLINE AND ACETYLCHOLINE BASED ON A GLASSY CARBON ELECTRODE MODIFIED BY PLATINUM-BLACK PARTICLES AND A CO-CROSSLINKED ENZYME SYSTEM

• Published in: Analytical letters 2001-02, Vol.34 (4), p.491-501
• Main Authors: Chen, Jun, Lin, Xiang-Qin, Chen, Zong-Hai, Wu, Shou-Guo, Wang, Shu-Qing
• Format: Article
• Language: English
• Subjects: Acetylcholine; Acetylcholineasterase; Amperometry; Biological and medical sciences; Biosensors; Biotechnology; Choline; Choline oxidase; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Micro-biosensor array; Platinum-black; Various methods and equipments
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1081/AL-100002590

A glassy carbon electrode was first modified by electro-depositing sub-micrometer platinum black particles on the surface. An acetylcholine micro-biosensor array and a choline micro-biosensor array were fabricated based on immobilization of acetylcholineasterase-choline oxidase or choline oxidase by cross-linking with glutaraldehyde on the Pt-black/GCE. Significant enhancement in performances of these biosensors was achieved. The chronoamperometric response of the acetylcholine micro-biosensor array gave a linear relation in the range of 29 to 1200μmol/L and a detection limit of 8.7μmol/L acetylcholine. The amperometric response of the choline micro-biosensor array gave a linear relation in the range of 24 to 1000μmol/L and a detection limit of 7.4μmol/L choline. They also possessed very good stability and reproducibility for long-term use.