Enzyme Biosensor for Tomatine Detection in Tomatoes

A biosensor for detection of tomatine in tomatoes has been developed using pH-sensitive field effect transistor as transducer and immobilised enzyme butyryl cholinesterase (BuChE) as a biorecognition element. The main analytical characteristics of the biosensor developed were studied for different c...

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Bibliographic Details
Published in:Analytical letters 2004-01, Vol.37 (8), p.1611-1624
Main Authors: Dzyadevych, Sergei V., Arkhypova, Valentyna N., Soldatkin, Alexey P., El'skaya, Anna V., Martelet, Claude, Jaffrezic-Renault, Nicole
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Electric power
Engineering Sciences
Enzyme biosensor
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Glycoalkaloid
Methods. Procedures. Technologies
Tomatine
Tomato
Various methods and equipments
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Summary:A biosensor for detection of tomatine in tomatoes has been developed using pH-sensitive field effect transistor as transducer and immobilised enzyme butyryl cholinesterase (BuChE) as a biorecognition element. The main analytical characteristics of the biosensor developed were studied for different conditions. The possibility to optimise these working parameters was investigated. By using this biosensor and enzyme inhibition effect, the tomatine can be measured in the concentration range of 0.5-50 µM with a detection limit of 0.2 µM. Tomatine concentrations in different tomato juice samples were determined by such a biosensor, and a good correlation with the known real content was revealed. A high reproducibility and operational stability of the biosensor developed were shown.
ISSN:0003-2719
1532-236X
DOI:10.1081/AL-120037591