GLOBIN CHAIN ANALYSIS BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY: RECENT DEVELOPMENTS

Reversed phase high performance liquid chromatography of globin chains is an important additional tool in the study of hemoglobin abnormalities. Using a technique modified from that of Leone et al.,[[1]] we report here the relative chromatographic behavior of about 200 different hemoglobin variants....

Full description

Saved in:
Bibliographic Details
Published in:Hemoglobin 2002-01, Vol.26 (3), p.271-283
Main Authors: Wajcman, Henri, Riou, Jean, Yapo, Angoué P.
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - trends
Genetic Variation
Globins - analysis
Globins - genetics
Hemoglobins - analysis
Hemoglobins - genetics
Hemoglobins, Abnormal - analysis
Hemoglobins, Abnormal - genetics
Humans
Thalassemia - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reversed phase high performance liquid chromatography of globin chains is an important additional tool in the study of hemoglobin abnormalities. Using a technique modified from that of Leone et al.,[[1]] we report here the relative chromatographic behavior of about 200 different hemoglobin variants. This method provides an additional dimension in the presumptive characterization of hemoglobin variants. It was also found to be of special value for measuring the expression of neutral variants, such as thalassemic or unstable hemoglobins, and to identify neutral mutations associated with another variant, resulting in unusual hematological presentations.
ISSN:0363-0269
1532-432X
DOI:10.1081/HEM-120015031