LIQUID CHROMATOGRAPHY DETERMINATION AND LC-ESI-MS ANALYSIS OF MICROCYSTIN-LR FROM PCC STRAINS OF MICROCYSTIS AERUGINOSA

Cyanobacteria (blue-green algae) have been shown to produce unique biologically active peptides such as microcystins, mainly from different species of Microcystis aeruginosa. Presence of these toxic peptides (hepatotoxin) in fresh water supplies are of increasing concern and has emphasized the need...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2002-01, Vol.25 (6), p.865-875
Main Authors: Nivsarkar, Manisha, Kaushik, M. P.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbial ecology
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Various environments (extraatmospheric space, air, water)
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Summary:Cyanobacteria (blue-green algae) have been shown to produce unique biologically active peptides such as microcystins, mainly from different species of Microcystis aeruginosa. Presence of these toxic peptides (hepatotoxin) in fresh water supplies are of increasing concern and has emphasized the need for a suitable analytical method. Amounts of microcystin-LR were estimated in the laboratory cultures of PCC strains viz. PCC-7806, 7820, and PCC-7941, using high performance liquid chromatography. The identity of microcystin-LR was confirmed by LC-ESI-MS. The lower detection limit of quantification is 1.0 μg/L.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-120003265