Quantitative Determination of Lanatosides in the Hybrid Digitalis ambigua × Digitalis lanata Leaves by HPLC

The primary glycosides in the hybrid Digitalis ambigua × Digitalis lanata leaves were determined by HPLC and compared with the measurement of lanatosides in D. ambigua and D. lanata leaves. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep-Pak cartridges prior to...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2003-01, Vol.26 (12), p.2013-2021
Main Authors: Ikeda, Yukari, Fujii, Youichi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cardiac glycoside
General pharmacology
HPLC
Hybrid Digitalis ambigua × Digitalis lanata
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Primary glycoside
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Summary:The primary glycosides in the hybrid Digitalis ambigua × Digitalis lanata leaves were determined by HPLC and compared with the measurement of lanatosides in D. ambigua and D. lanata leaves. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep-Pak cartridges prior to HPLC analysis. HPLC was performed on a TSK-GEL AMIDE-80 column using acetonitril:water (96:4). The effluent was monitored by ultraviolet (UV) absorption at 220 nm. The quantitation was carried out by the internal standard method. The amount of lanatoside C per 100 mg of dry leaf powder samples of the hybrid D. ambigua × D. lanata and D. lanata were 227.3 µg and 134.4 µg, respectively. The amount of lanatoside C in the hybrid D. ambigua × D. lanata was higher than in D. lanata.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-120021768