Determination of thujone by derivatization with dansylhydrazine and liquid chromatography

The reaction of α- and β-thujones with dansylhydrazine (DNSH) was studied using reversed phase liquid chromatography (LC) with fluorescence detection. The derivatization procedure, although slow, was applied for determination of α-thujone in white vermouth at concentrations 0.45-4.5 µg/L using a C 1...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2004, Vol.27 (6), p.1071-1081
Main Authors: Scott, P.M, Lawrence, G.A, Lau, B.P.Y
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
chemical analysis
Chemistry
Chromatographic methods and physical methods associated with chromatography
Dansylhydrazine
essential oils
Exact sciences and technology
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
Liquid chromatography
Other chromatographic methods
Thujone
Vermouth
Wines and vinegars
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Summary:The reaction of α- and β-thujones with dansylhydrazine (DNSH) was studied using reversed phase liquid chromatography (LC) with fluorescence detection. The derivatization procedure, although slow, was applied for determination of α-thujone in white vermouth at concentrations 0.45-4.5 µg/L using a C 18 solid phase extraction column for cleanup; recoveries of added α-thujone were in the range 60-77%. Separation of dansyl α- and β-thujones was achieved by LC and their identity confirmed by LC-tandem mass spectrometry (MS/MS).
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-120030179