Comparison of fluorescence immunochromatography and HPLC for the trace analysis of algal toxins

New monoclonal antibodies (mAbs) against microcystin (MC), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa, were prepared from cloned hybridoma cell lines. Using these mAbs, an immunochromatographic assay system was designed for the detection of cyanobacterial hepatoto...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2004, Vol.27 (20), p.3189-3202
Main Authors: Pyo, D.J, Choi, J.C, Lim, C.H, Choi, E.Y
Format: Article
Language:English
Subjects:
Analytical chemistry
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Cyanobacterium
Detection
Ecotoxicology, biological effects of pollution
Exact sciences and technology
fluorescent antibody technique
Fresh water environment
Freshwater
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
HPLC
Immunochromatography
Microcystins
Microcystis aeruginosa
Monoclonal antibodies
Other chromatographic methods
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Summary:New monoclonal antibodies (mAbs) against microcystin (MC), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa, were prepared from cloned hybridoma cell lines. Using these mAbs, an immunochromatographic assay system was designed for the detection of cyanobacterial hepatotoxins, MCs in water. The immunochromatographic assay system is composed of anti-MCs-mAb, fluorescence conjugates in detection solution, an immunochromatographic assay strip, and a laser fluorescence scanner. The performance of the immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. As the detection limit of HPLC is several orders of magnitude higher than with immunochromatographic assay systems, attention was also given to preconcentration of samples with solid phase extraction cartridges.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-200034876