Reversed Phase Liquid Chromatographic Method for the High-Throughput Analysis of Clopidogrel in Pharmaceutical Formulations Using a Monolithic Silica Column

A high-throughput, high performance liquid chromatographic method was developed and validated for the determination of clopidogrel in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed phase monolithic silica column Chromolith Performance 18e (100 mm×4.6 mm...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2005-05, Vol.28 (9), p.1357-1365
Main Authors: Aboul-Enein, Hassan Y., Hoenen, Hubert, Ghanem, Ashraf, Koll, Michael
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Clopidogrel
Exact sciences and technology
General pharmacology
High performance liquid chromatography
HPLC
Medical sciences
Monolithic column
Other chromatographic methods
Pharmaceutical analysis
Pharmacology. Drug treatments
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Summary:A high-throughput, high performance liquid chromatographic method was developed and validated for the determination of clopidogrel in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed phase monolithic silica column Chromolith Performance 18e (100 mm×4.6 mm I.D.). The mobile phase consisted of acetonitrile:phosphate buffer (50∶50 v/v, pH 3.0) at a flow rate of 4.0 mL/min. The photodiode array detector was set at 235 nm. The developed method showed a good linear relationship in the concentration range from 1.0 to 40.0 µg/mL with a correlation coefficient of 0.999. The limit of detection and limit of quantification were 0.97 µg/mL and 3.52 µg/mL, respectively.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-200054835