KINETICS STUDY OF THE BIOTRANSFORMATION OF AN OLIGONUCLEOTIDE PRODRUG IN CELLS EXTRACT BY MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY

The fate of a dodecathymidine prodrug in cell extract was monitored by MALDI-TOF MS. This technique allows a facile identification and a relative quantification of metabolites produced. We showed that the relative peak intensities were similar to the relative metabolite proportions that permitted th...

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Published in:Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2001-04, Vol.20 (4-7), p.1159-1163
Main Authors: Morvan, François, Brès, Jean-Charles, Lefebvre, Isabelle, Vasseur, Jean-Jacques, Pompon, Alain, Imbach, Jean-Louis
Format: Article
Language:English
Subjects:
Biotransformation
Oligonucleotides - pharmacokinetics
Prodrugs - pharmacokinetics
Pyrimidine Nucleotides - pharmacokinetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Thymidine - analogs & derivatives
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creator Morvan, François
Brès, Jean-Charles
Lefebvre, Isabelle
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Pompon, Alain
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description The fate of a dodecathymidine prodrug in cell extract was monitored by MALDI-TOF MS. This technique allows a facile identification and a relative quantification of metabolites produced. We showed that the relative peak intensities were similar to the relative metabolite proportions that permitted the determination of their half-lives. The oligonucleotide prodrug was fully metabolized to yield the T 12 phosphorothioate likely through a carboxyesterase mediated mechanism.
doi_str_mv 10.1081/NCN-100002510
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source Taylor and Francis Science and Technology Collection
subjects Biotransformation
Oligonucleotides - pharmacokinetics
Prodrugs - pharmacokinetics
Pyrimidine Nucleotides - pharmacokinetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Thymidine - analogs & derivatives
title KINETICS STUDY OF THE BIOTRANSFORMATION OF AN OLIGONUCLEOTIDE PRODRUG IN CELLS EXTRACT BY MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY
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