3′-MODIFIED OLIGO(2′-O-METHYLRIBONUCLEOTIDES) AS IMPROVED PROBES FOR HYBRIDIZATION WITH RNA

• Published in: Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2005-01, Vol.24 (5-7), p.527-531
• Main Authors: Novopashina, D. S., Totskaya, O. S., Lomzov, A. A., Venyaminova, A. G.
• Format: Article
• Language: English
• Subjects: 3′-Dangling "Inverted" Deoxyribonucleoside; Base Pair Mismatch; Base Sequence; Deoxyribonucleotides - chemistry; DNA - chemistry; DNA, Complementary - metabolism; Duplex Stability; Kinetics; Models, Chemical; Molecular Sequence Data; Nuclease Resistance; Nucleic Acid Conformation; Nucleic Acid Heteroduplexes; Nucleic Acid Hybridization; Oligo(2′-O-methylribonucleotides); Oligonucleotides - chemistry; Oligoribonucleotides - chemistry; Ribonucleotides - chemistry; RNA - chemistry; RNA, Complementary - chemistry; Temperature; Thermodynamics
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1081/NCN-200061795

A series of octa(2′-O-methylribonucleotides) with an additional 3′-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3′-3′ ("inverted") bond was synthesized. The exceptional stability of these oligomers to a 3′-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3′-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2′-O-methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal A m U(T) pair and enhances the mismatch sensitivity.