DIFFERENT SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF CIMETIDINE, RANITIDINE HYDROCHLORIDE, AND FAMOTIDINE

• Published in: Spectroscopy letters 2002-08, Vol.35 (4), p.543-563
• Main Authors: Kelani, Kahdiga M., Aziz, Azza M., Hegazy, Maha A., Fattah, Laila Abdel
• Format: Article
• Language: English
• Subjects: CColorimetric methods; Cimetidine; Famotidine; Ranitidine hydrochloride; Spectrophotometry; Stability indicating methods
• ISSN: 0038-7010; 1532-2289
• DOI: 10.1081/SL-120013890

In this work three different spectrophotometric methods were established for the determination of cimetidine (I), ranitidine hydrochloride (II) and famotidine (III) The first one is a colorimetric method, it was applied for the determination of the three drugs by using sodium nitroprusside as a color reagent to produce a red colored complexes. In this method, the zero order spectrum 0 D was used for the determination of drug (III) at λ =500 nm while the first derivative spectra 1 D were used for the determination of drug (I) and (II) at their corresponding wave lengths 523 and 510 nm and Δ λ =4 nm. The method can be considered as a stability indicating method for the determination of the three drugs in the presence of their induced hydrogen peroxide oxidative degradates. Beer's law was obeyed in the concentration range of 25-150 µg·mL −1 for (I) and 50-500 µg·mL −1 for (II) and (III) with mean percentage recoveries of 100.27±0.697, 99.79±0.465 and 99.15±0.687, respectively. The second method is a simple colorimetric method, it was applied for the determination of drug (III). Where, 3-Methyl-2-benzo-thiazolinone hydrazone (MBTH) was used as a color reagent. It reacts with the drug to produce a bluish violet color, having two maxima at 536 and 620 nm. The percentage recoveries were 99.73±1.048 and 99.94±0.887, respectively within the concentration range of 20-120 µg·mL −1 . The third one is a spectrophotometric method via a complex formation reaction by using cobalt II. A colorless complex was developed having λ max at 319 nm with a ratio of 1:1 and a stability constant logarithm of 5.49. The percentage recovery was 99.84±0.858 within a concentration range of 10-60 µg·mL −1 . The statistical comparison with the BP official methods [1] and the assay validation for the three proposed methods has been applied. The results obtained showed that they could be used for the determination of the three drugs in pure and dosage forms.