Biological Control of Aflatoxin Contamination of Crops

• Published in: Journal of toxicology. Toxin reviews 2004-01, Vol.23 (2-3), p.425-450
• Main Author: Dorner, Joe W
• Format: Article
• Language: English
• Subjects: AF36; Afla-guard; aflatoxins; Arachis hypogaea; Aspergillus flavus; Aspergillus parasiticus; Biocontrol; biological control; biological control agents; Corn; Cotton; Food safety; Formulation competitive exclusion; fungal antagonists; fungal diseases of plants; Gossypium; Gossypium hirsutum; Groundnuts; literature reviews; Maize; microbial contamination; Peanuts; Zea mays
• ISSN: 0731-3837; 1525-6057
• DOI: 10.1081/TXR-200027877

Aflatoxin contamination of crops compromises the safety of food and feed supplies and causes significant economic losses each year. Of the many research approaches being studied to reduce and, ultimately, eliminate aflatoxin contamination, biological control is one of the more promising, particularly for the near‐term. Numerous organisms have been tested for biological control of aflatoxin contamination including bacteria, yeasts, and nontoxigenic strains of the causal organisms, Aspergillus flavus and A. parasiticus. Most of the field successes to date have been achieved by applying certain nontoxigenic strains of A. flavus and A. parasiticus to soil of susceptible crops, such as peanuts, cotton, and corn. The applied strains occupy the same niche as the naturally occurring toxigenic strains and competitively exclude them when crops are susceptible to infection. Various formulations have been used to apply the nontoxigenic strains to soil, but the most effective methods have been to combine the desired strain with a carrier/substrate, such as a small grain. This was done either by minimally growing the desired strain on sterilized grain or by coating the surface of the grain with conidia of the strain. After application to the field and uptake of moisture, the fungus completely colonizes the grain, and abundant sporulation provides inoculum levels sufficient to achieve a competitive advantage for the nontoxigenic strain. In several years of field studies, particularly with peanuts and cotton, significant reductions in aflatoxin contamination in the range of 70-90% have been achieved consistently. Two separate products have recently received EPA registration as biopesticides to control aflatoxin contamination in cotton (AF36) and peanuts (afla‐guard).