Metallothionein Induction in Early Larval Stages of Tilapia (Oreochromis mossambicus)

• Published in: Physiological and biochemical zoology 2000-09, Vol.73 (5), p.531-537
• Main Authors: Wu, Su‐Mei, Weng, Ching‐Feng, Hwang, Ji‐Chuu, Huang, Chang‐Jen, Hwang, Pung‐Pung
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Cadmium - adverse effects; Fish larvae; Hatching; Heavy metals; Larva - drug effects; Larva - growth & development; Larvae; Larval development; Messenger RNA; Metallothionein - biosynthesis; Tables of contents; Tap water; Tilapia; Tilapia - physiology; Water Pollutants, Chemical - adverse effects
• ISSN: 1522-2152; 1537-5293
• DOI: 10.1086/317754

Amounts of whole‐body metallothionein (MT) in tilapia (Oreochromis mossambicus) larvae increased to a peak (1,500 ng mg−1protein) 1 d after hatching (H1), decreased rapidly thereafter, and was maintained at a constant level (700 ng mg−1) 3 d after hatching (H3). Waterborne Cd2+could stimulate MT expression in newly hatched (H0) larvae in dose‐dependent and time‐dependent patterns. H0 larvae, which were treated with 35 μg L−1Cd2+for 24 h, showed a 1.7‐fold increase in the MT amount ( \documentclass{aastex} \usepackage{amsbsy} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{bm} \usepackage{mathrsfs} \usepackage{pifont} \usepackage{stmaryrd} \usepackage{textcomp} \usepackage{portland,xspace} \usepackage{amsmath,amsxtra} \usepackage[OT2,OT1]{fontenc} \newcommand\cyr{ \renewcommand\rmdefault{wncyr} \renewcommand\sfdefault{wncyss} \renewcommand\encodingdefault{OT2} \normalfont \selectfont} \DeclareTextFontCommand{\textcyr}{\cyr} \pagestyle{empty} \DeclareMathSizes{10}{9}{7}{6} \begin{document} \landscape $174.0\pm 64.7$ \end{document} ) and a 6.5‐fold increase in accumulated Cd2+but no significant change in Ca2+content, compared with the H0 control (MT, \documentclass{aastex} \usepackage{amsbsy} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{bm} \usepackage{mathrsfs} \usepackage{pifont} \usepackage{stmaryrd} \usepackage{textcomp} \usepackage{portland,xspace} \usepackage{amsmath,amsxtra} \usepackage[OT2,OT1]{fontenc} \newcommand\cyr{ \renewcommand\rmdefault{wncyr} \renewcommand\sfdefault{wncyss} \renewcommand\encodingdefault{OT2} \normalfont \selectfont} \DeclareTextFontCommand{\textcyr}{\cyr} \pagestyle{empty} \DeclareMathSizes{10}{9}{7}{6} \begin{document} \landscape $102.6\pm 48.1$ \end{document} ). H3 larvae with the same treatment revealed about a 10‐fold increase in accumulated Cd2+, a 10% decrease in Ca2+content, but no change in MT ( \documentclass{aastex} \usepackage{amsbsy} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{bm} \usepackage{mathrsfs} \usepackage{pifont} \usepackage{stmaryrd} \usepackage{textcomp} \usepackage{portland,xspace} \usepackage{amsmath,amsxtra} \usepackage[OT2,OT1]{fontenc} \newcommand\cyr{ \renewcommand\rmdefault{wncyr} \renewcommand\sfdefault{wncyss} \renewcommand\encodingdefault{OT2} \normalfont \selectfont} \DeclareTextFontCommand{\textcyr}{\cyr} \pagestyle{empty} \DeclareMathSizes{10}{9}{7}{6} \begin{document} \landscape $261.2\pm 120.0$ \end{document} ), compared with the H3 control (MT, \documentclass{a