Differentiation of plantlets in longleaf pine (Pinus palustris Mill.) tissue cultured in vitro

Embryos of longleaf pine (Pinus palustris Mill.) were induced to form numerous adventitious buds along their cotyledons when placed in culture on chemically defined nutrient media. The buds when excised and transferred to other media form roots and vigorous plantlets. Plantlets were obtained from nu...

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Bibliographic Details
Published in:Botanical gazette (Chicago, Ill.) Ill.), 1975-06, Vol.136 (2), p.196-200
Main Authors: Sommer, Harry E., Brown, Claud L., Kormanik, Paul P.
Format: Article
Language:English
Subjects:
Apical meristems
Biota
Callus
Cotyledons
Embryos
Histology
Plant roots
Plantlets
Root meristems
Tap roots
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Summary:Embryos of longleaf pine (Pinus palustris Mill.) were induced to form numerous adventitious buds along their cotyledons when placed in culture on chemically defined nutrient media. The buds when excised and transferred to other media form roots and vigorous plantlets. Plantlets were obtained from numerous embryos using three different seed sources. Histological observations describing these patterns of differentiation are presented.
ISSN:0006-8071
1940-1205
DOI:10.1086/336802