Laser speckle imaging of blood flow in microcirculation

Monitoring the spatio-temporal characteristics of microcirculation is crucial for studying the functional activities of biotissue and the mechanism of disease. However, conventional methods used to measure blood flow suffer from limited spatial resolution or the injection of exogenous substances or...

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Bibliographic Details
Published in:Physics in medicine & biology 2004-04, Vol.49 (7), p.1347-1357
Main Authors: Cheng, Haiying, Luo, Qingming, Liu, Qian, Lu, Qiang, Gong, Hui, Zeng, Shaoqun
Format: Article
Language:English
Subjects:
Animals
Antihypertensive Agents - pharmacology
Blood Flow Velocity - drug effects
Blood Flow Velocity - physiology
Dose-Response Relationship, Drug
Image Interpretation, Computer-Assisted - methods
Laser-Doppler Flowmetry - methods
Male
Mesentery - blood supply
Mesentery - cytology
Mesentery - drug effects
Microcirculation - cytology
Microcirculation - drug effects
Microcirculation - physiology
Phentolamine - pharmacology
Rats
Rats, Wistar
Splanchnic Circulation - physiology
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Summary:Monitoring the spatio-temporal characteristics of microcirculation is crucial for studying the functional activities of biotissue and the mechanism of disease. However, conventional methods used to measure blood flow suffer from limited spatial resolution or the injection of exogenous substances or the need of scanning to obtain the dynamic of regional blood flow. Laser speckle imaging (LSI) technique makes up these disadvantages by obtaining the regional blood flow distribution with high spatio-temporal resolution without the need to scan. In this paper, LSI was introduced to investigate the dynamic responses of the rat mesenteric microcirculation to an incremental dose of phentolamine. The results showed that when the dose of phentolamine was less than 4 microg ml(-1), local application of phentolamine on the mesentery would increase the blood perfusion as the concentration increased. When the dose increased further, the improvement decreased. At a dose of 200 microg ml(-1), a microcirculation impediment was caused. At the same time, different responses between veinules and arterioles were manifested. These suggested that LSI is promising to be a useful contribution to drug development and testing.
ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/49/7/020