Ultrasensitive label-free DNA analysis using an electronic chip based on carbon nanotube nanoelectrode arrays

We report the detection of DNA PCR amplicons using an ultrasensitive label-free electronic technique based on multiwalled carbon nanotube (MWNT) nanoelectrode arrays embedded in an SiO(2) matrix. Specific PCR amplicons are reliably detected using electrochemical (EC) methods through allele-specific...

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Bibliographic Details
Published in:Nanotechnology 2003-12, Vol.14 (12), p.1239-1245
Main Authors: Koehne, Jessica, Chen, Hua, Li, Jun, Cassell, Alan M, Ye, Qi, Ng, Hou Tee, Han, Jie, Meyyappan, M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Applied sciences
Biological and medical sciences
Cross-disciplinary physics: materials science
rheology
Electronics
Enzymes and enzyme inhibitors
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Interfaces
Materials science
Nanoscale materials and structures: fabrication and characterization
Nanotubes
Physics
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
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Summary:We report the detection of DNA PCR amplicons using an ultrasensitive label-free electronic technique based on multiwalled carbon nanotube (MWNT) nanoelectrode arrays embedded in an SiO(2) matrix. Specific PCR amplicons are reliably detected using electrochemical (EC) methods through allele-specific oligonucleotide hybridization. The inherent guanine bases in the DNA amplicon target of [Formula: see text] bases serve as signal moieties with the aid of Ru(bpy)(3)(2+) mediators, providing an amplified anodic current associated with the oxidation of guanine groups at the nanoelectrode surface. The reduced size and density of the nanoelectrode array provided by MWNTs dramatically improves the sensitivity of EC detection. In addition, the abundant guanine bases in target DNA produce a large signal. Less than [Formula: see text] target amplicons can be detected on a microspot, approaching the sensitivity limit of conventional laser-based fluorescence techniques. This method also eliminates the labelling requirement and makes the measurements much simpler. This platform can be employed for developing highly automated electronic chips with multiplex nanoelectrode arrays for quick DNA analysis.
ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/14/12/001