Cyclodextrin nanoaggregates and their assembly with protein: a spectroscopic investigation

Light scattering and time-resolved fluorescence spectroscopy results showed that specially designed amphiphilic cyclodextrins are able to bind a specific protein, PA-I lectin. When containing a galactosyl group, the self-assembled cyclodextrins interact with the protein affecting the dynamical prope...

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Bibliographic Details
Published in:Nanotechnology 2006-07, Vol.17 (13), p.3239-3244
Main Authors: Micali, N, Villari, V, Mazzaglia, A, Monsú Scolaro, L, Valerio, A, Rencurosi, A, Lay, L
Format: Article
Language:English
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Summary:Light scattering and time-resolved fluorescence spectroscopy results showed that specially designed amphiphilic cyclodextrins are able to bind a specific protein, PA-I lectin. When containing a galactosyl group, the self-assembled cyclodextrins interact with the protein affecting the dynamical properties of the system and the fluorescence lifetimes (as well as the fluorescence anisotropy) of the protein itself. The self-assembled cyclodextrins containing a glucosyl group, on the other hand, do not induce any change in these measured quantities, suggesting no interaction with protein. This binding capability of galactosyl-modified cyclodextrins offers perspectives on exploiting self-assembled supramolecular structures as nano-carriers to deliver drugs to target tissues.
ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/17/13/027