Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C60) nanoparticles

The present study investigated the hemolytic properties of fullerene (C(60)) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl ace...

Full description

Saved in:
Bibliographic Details
Published in:Nanotechnology 2010-09, Vol.21 (37), p.375102-375102
Main Authors: Trpkovic, Andreja, Todorovic-Markovic, Biljana, Kleut, Duska, Misirkic, Maja, Janjetovic, Kristina, Vucicevic, Ljubica, Pantovic, Aleksandar, Jovanovic, Svetlana, Dramicanin, Miroslav, Markovic, Zoran, Trajkovic, Vladimir
Format: Article
Language:English
Subjects:
Buckminsterfullerene
Erythrocytes
Erythrocytes - cytology
Ethylene
Fullerenes
Fullerenes - adverse effects
Fullerenes - chemistry
Furans - adverse effects
Furans - chemistry
gamma-Cyclodextrins - adverse effects
gamma-Cyclodextrins - chemistry
Hemoglobin
Hemolysis
Human
Humans
Nanoparticles
Nanoparticles - adverse effects
Nanoparticles - chemistry
Oxidative Stress
Polyvinyls - adverse effects
Polyvinyls - chemistry
Serum Albumin - metabolism
Solvents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study investigated the hemolytic properties of fullerene (C(60)) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose- and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.
ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/21/37/375102