From in vitro to ex vivo: subcellular localization and uptake of graphene quantum dots into solid tumors

• Published in: Nanotechnology 2019-09, Vol.30 (39), p.395101
• Main Authors: Kersting, David, Fasbender, Stefan, Pilch, Rabea, Kurth, Jennifer, Franken, André, Ludescher, Marina, Naskou, Johanna, Hallenberger, Angelika, Gall, Charlotte von, Mohr, Corinna J, Lukowski, Robert, Raba, Katharina, Jaschinski, Sandra, Esposito, Irene, Fischer, Johannes C, Fehm, Tanja, Niederacher, Dieter, Neubauer, Hans, Heinzel, Thomas
• Format: Article
• Language: English
• Subjects: Breast Neoplasms - metabolism; Breast Neoplasms - pathology; cellular uptake; drug delivery; Epithelial Cells - metabolism; graphene quantum dots; Graphite - chemistry; Graphite - metabolism; Humans; intracellular localization; Nanostructures - chemistry; Particle Size; Quantum Dots - chemistry; Quantum Dots - metabolism; Subcellular Fractions - metabolism; Tissue Culture Techniques; tissue slices; Tumor Cells, Cultured
• ISSN: 0957-4484; 1361-6528
• DOI: 10.1088/1361-6528/ab2cb4

Among various nanoparticles tested for pharmacological applications over the recent years, graphene quantum dots (GQDs) seem to be promising candidates for the construction of drug delivery systems due to their superior biophysical and biochemical properties. The subcellular fate of incorporated nanomaterial is decisive for transporting pharmaceuticals into target cells. Therefore a detailed characterization of the uptake of GQDs into different breast cancer models was performed. The demonstrated accumulation inside the endolysosomal system might be the reason for the particles' low toxicity, but has to be overcome for cytosolic or nuclear drug delivery. Furthermore, the penetration of GQDs into precision-cut mammary tumor slices was studied. These constitute a far closer to reality model system than monoclonal cell lines. The constant uptake into the depth of the tissue slices underlines the systems' potential for drug delivery into solid tumors.