Ethanolic leaf extracts of Waru (Hibiscus tiliaceus) inhibit biofilm formation of Vibrio alginolyticus in vitro

• Published in: Journal of physics. Conference series 2019-10, Vol.1321 (3), p.32045
• Main Authors: Suhartono, S, Fitri, L, Nurhaliza, N, Ismail, Y S, Nursanty, R, Mahyuddin, M, Jannah, M
• Format: Article
• Language: English
• Subjects: Aquaculture; Bacteria; Biofilms; Optical density; Physics
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1321/3/032045

Vibriosis is still a major threat in aquaculture generating significant implications in ecology and economy. One of the ethologic agents of vibriosis is Vibrio alginolyticus that infects shrimp and fish aquaculture through biofilm-mediated. The objective of the study was to determine the biopotency of ethanolic extract of waru (Hibiscus tiliaceus) leaves to inhibit biofilm formation of V. alginolyticus in vitro. A microtiter plate biofilm assay (OD570) method was applied in this study. The treatments were the addition of ethanolic leaves extracts of waru (H. tiliaceus) at different concentrations of 2%, 4%, 8%, and 10%. The values of biofilm inhibition activity were measured as optical density data that were statistically analyzed using One Way ANOVA, followed by Tukey's test and the tests were considered statistically significant at a P ≤ 0.05 on two-tailed. The result of this study showed that Waru leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The best inhibition was shown at 10% concentration of extract signifying the potential application of waru to treat biofilm-mediated diseases in aquaculture.